The multiple antibiotic resistant (MAR) index and detection of resistant genes in the bacterial consortium of urine samples collected from University of Medical Sciences Teaching Hospital,Akure (UNIMEDTH) was evaluated with all microbiological and biotechnological techniques employed utilizing specified standards in this study. Escherichia coli had the highest bacterial count (311.50 ± 0.707 CFU/ml) while Staphylococcus saprophyticus had the least (13.00 ± 2.828 CFU/ml). Enterococcus faecalis, and Pseudomonas aeruginosa isolate showed marked resistance against four classes of antibiotics tested
